FITC标记的mRNA cap甲基转移酶抗体
产品名称： FITC标记的mRNA cap甲基转移酶抗体
英文名称： Anti-HCM/FITC
产品编号： HZ-15427R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-HCM/FITC Conjugated antibody
FITC标记的mRNA cap甲基转移酶抗体
| 英文名称 | Anti-HCM/FITC |
| 中文名称 | FITC标记的mRNA cap甲基转移酶抗体 |
| 别 名 | RNMT; DKFZp686H1252; EC 2.1.1.56; hcm1p; hCMT1; HCMT1a; hCMT1c; hMet; hypothetical protein KIAA0396; KIAA0398; MCES_HUMAN; MET; mRNA (guanine 7)methyltransferase; mRNA (guanine N(7)) methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap guanine-N7 methyltransferase; mRNA cap methyltransferase; OTTHUMP00000162509; RG7MT1; RNA (guanine 7) methyltransferase; RNA guanine 7 methyltransferase; Rnmt. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HCM |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: RNMT (RNA (guanine-7-) methyltransferase) is a widely expressed nuclear protein that belongs to the mRNA cap methyltransferase family. It is responsible for catalyzing the final step in the attachment of the m7GpppN cap to the 5' end of mRNA. Capping of mRNA plays an important role in mRNA processing, stability and translation and is therefore important for efficient gene expression. There are three enzymatic steps in the generation of the mRNA cap. The first two steps are catalyzed by RNGTT (RNA guanylyltransferase and 5' phosphatase), and the third step is catalyzed by RNMT. More specifically, RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thereby producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA. Two additional isoforms of RNMT exist due to alternative splicing events, namely hCMT1a and hCMT1b. Function: mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC. Subunit: Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates. Interacts with elongating form of polymerase II and RNGTT. Interacts with RAM/FAM103A1, the interaction significantly enhances RNA-binding and cap methyltransferase activity. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed. Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. Contains 1 mRNA cap 0 methyltransferase domain. Database links: Entrez Gene: 8731 Human Omim: 603514 Human SwissProt: O43148 Human Unigene: 592347 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
RNMT(RNA(鸟嘌呤-7-)甲基转移酶)是一种广泛表达的核蛋白，属于mRNA帽甲基转移酶家族。它负责催化M7GPPPN帽附着到mRNA的5’端的最后步骤。mRNA的封端在mRNA的加工、稳定性和翻译中起着重要作用，因此对于有效的基因表达具有重要意义。在mRNA的产生过程中有三个酶的步骤。前两步由RNGTT（RNA鸟苷酰基转移酶和5’磷酸酶）催化，第三步由RNMT催化。更具体地说，RNMT催化甲基从AdoMet(S-腺苷蛋氨酸)转移到N-7位生长mRNA的GpppN端，从而产生AdoHyc(S-腺苷同型半胱氨酸)和m7GpppN终止RNA。由于选择性剪接事件，即HCMT1A和HCMT1B，RNMT的两个额外的异构体存在。