FITC标记的β防御素3抗体
产品名称： FITC标记的β防御素3抗体
英文名称： Anti-beta Defensin 3/FITC
产品编号： HZ-7378R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:200-800
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Rabbit Anti-beta Defensin 3/FITC Conjugated antibody
FITC标记的β防御素3抗体
| 英文名称 | Anti-beta Defensin 3/FITC |
| 中文名称 | FITC标记的β防御素3抗体 |
| 别 名 | BD 3; BD-3; BD3; beta 103; Beta defensin 103A; Beta defensin 103A precursor; Beta defensin 3; Beta-defensin 103; Beta-defensin 3; D103A_HUMAN; DEF B3; DEFB 103; DEFB 103A; DEFB 3; DEFB-3; DEFB103; DEFB103A; DEFB103B; DEFB3; Defensin; Defensin beta 103A; Defensin beta 3; Defensin like protein; Defensin-like protein; HBD 3; hBD-3; HBD3; HBP 3; HBP3; mBD3. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:200-800 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 5kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human beta Defensin 3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed. Subcellular Location: Secreted. Tissue Specificity: Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine. Similarity: Belongs to the beta-defensin family. Database links: Entrez Gene: 414325 Human Entrez Gene: 55894 Human Omim: 606611 Human SwissProt: P81534 Human Unigene: 283082 Human Unigene: 637221 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.  Paraformaldehyde-fixed, paraffin embedded (Human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.  Blank control(blue): Hep G2 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-beta Defensin 3/FITC Conjugated antibody (bs-7378R-FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions |
对革兰氏阳性细菌、金黄色葡萄球菌、化脓性葡萄球菌、革兰氏阴性细菌、铜绿假单胞菌、大肠杆菌和白色念珠菌均有抗菌活性。杀死多重耐药S.aureus和万古霉素抗性屎肠球菌。未观察到明显的溶血活性。