Description

 

The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction [1], cardiac fibrosis, and is often complicated by cardiac hypertrophy [2]. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression [3]. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors.

HCFav from ScienCell Research Laboratories are isolated from the human heart. HCF-av are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCFav are characterized by immunofluorescent method with antibody to fibronectin. HCF-av are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-av are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium

 

It is recommended to use fibroblast medium (FM, Cat. No. 2301) for the culturing of HCF-av in vitro.

Product Use

 

HCF-av are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

 

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

 

Dry ice.

Reference

 

[1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2002) Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes. Circ. Res. 91:532-9.
[2] Akiyama-Uchida, Y. et al., (2002) Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension 40:148-54.
[3] Burgess, M. L., Terracio, L., Hirozane, T., Borg, T. K. (2002) Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. Cardiovasc Pathol 11(2):78-87.