Amaxa^®Nucleofector^®技术是 Lonza(原Amaxa)公司的专利创新技术，它综合应用传统的电穿孔技术及细胞特异性细胞核转染液，通过调整优化电转参数（内存转染程序），直接把外源基因导入原代细胞及细胞系的细胞浆和细胞核中。

外源基因直接导入核内

Normal human dermal fibroblasts –neonatal were nucleofected with 2.5 μg TMR-labeled plasmid DNA encoding eGFP. Fixation of cells was performed after 2 hours with 3.5 % PFA. TMR label is shown in (A), GFP fluorescence in (B), DAPI nuclear staining in (C) and a merge of all three fluorescent labels in (D).

最广泛细胞类型的高效转染

成功高效转染1200余种细胞系                       优化转染130余种原代细胞

维持细胞生理功能

Human H9 ES cells preserve pluripotency post  

维持细胞生理功能

Nucleofection. H9 cells were transfected by Nucleofection with the pmaxGFP Vector. (Left) Cells analyzed after 24 hours show expression of GFP (green) as well as of the pluri potency markers SSEA4 (red) and Oct4 (purple). The blue signals refer to nuclear staining by DAPI. (Right) The percentage of double-positive cells (GFP/SSEA) was analyzed by fl ow cytometry. (Data kindly provided by Jennifer Moore, Rutgers University, Piscataway, USA.)