生长关联蛋白43(GAP43)重组蛋白

YB327Ra011
Recombinant Growth Associated Protein 43 (GAP43)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
生长关联蛋白43(GAP43)重组蛋白[ PROPERTIES ]
Residues: Met1~Ala274 (Accession # P17677),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cell membrane;
Peripheral membrane protein; Cytoplasmic side.
Cell projection, growth cone
membrane; Cell junction, synapse.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
生长关联蛋白43(GAP43)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.2
Predicted Molecular Mass: 32.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
生长关联蛋白43(GAP43)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MTKSCSELCH PALHFLPCLG
GLRKNLQRAV RPSPYSLGFL TFWISRVEKN DDDQKIEQDG IKPEDKAHKA ATKIQASFRG
HITRKKLKGE KKDDVQAAEA EANKKDEAPV ADGVEKKGEG TTTAEAAPAT GSKPDEPGKA
GETPSEEKKG EGDAATEQAA PQAPASSEEK AGSAETESAT KASTDNSPSS KAEDAPAKEE
PKQADVPAAV TAAAATTPAA EDAAAKATAQ PPTETGESSQ AEENIEAVDE TKPKESARQD
EGKEEEPEAD QEHA