视网膜母细胞瘤蛋白1(RB1)重组蛋白

YB211Hu021
Retinoblastoma Retinoblastoma Retinoblastoma Retinoblastoma Protein Protein Protein Protein 1 (RB1)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
视网膜母细胞瘤蛋白1(RB1)重组蛋白NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
5th Edition (Revised in January, 2013)
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Retinoblastoma Protein 1
Synonyms: Synonyms: Synonyms: Synonyms: RB1
Species: Species: Species: Species: Human
Size: 100µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: 视网膜母细胞瘤蛋白1(RB1)重组蛋白Location: Nucleus.

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Ile753~Lys928 (Accession # P06400), with
N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 24kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 21.4kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.9
Human RB1 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
视网膜母细胞瘤蛋白1(RB1)重组蛋白Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-IVFYNSVF MQRLKTNILQ YASTRPPTLS PIPHIPRSPY KFPSSPLRIP
GGNIYISPLK SPYKISEGLP TPTKMTPRSR ILVSIGESFG TSEKFQKINQ MVCNSDRVLK
RSAEGSNPPK PLKKLRFDIE GSDEADGSKH LPGESKFQQK LAEMTSTRTR
MQKQKMNDSM DTSNKEEK