人高迁移率族核小体结合域蛋白1(HMGN1)多克隆抗体
Polyclonal Antibody to High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 500µg/mL
UOM: 100µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA.
[ IMMUNOGEN ]
Immunogen:Recombinant HMGN1 (Lys14~Asp100) e expressed in E.coli
[ APPLICATIONS ]
Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing
0.05% Proclin-300, 50% glycerol
[ STORAGE AND STABILITY ] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ IDENTIFICATION ]


Figure 1. Western Blot
A. Sample: Recombinant HMGN1, Human B. Lane1: Human HepG2 Cells Lane2: Human Hela Cells Primary Ab: 2µg/mL Rabbit Anti-Human HMGN1 Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。