FITC标记的自主生长因子1B抗体
产品名称： FITC标记的自主生长因子1B抗体
英文名称： Anti-GFI1B/FITC
产品编号： HZ-9881R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
- 二维码 : 点击查看
Rabbit Anti-GFI1B/FITC Conjugated antibody
FITC标记的自主生长因子1B抗体
| 产品编号 | bs-9881R-FITC |
| 英文名称 | Anti-GFI1B/FITC |
| 中文名称 | FITC标记的自主生长因子1B抗体 |
| 别 名 | Potential regulator of CDKN1A translocated in CML; GFI 1B; GFI-1B; gfi1b; GFI1B protein; GFI1B_HUMAN; Growth factor independent 1B protein; Growth factor independent protein 1B; Potential regulator of CDKN1A; Translocated in CML; Zinc finger protein Gfi-1b. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 心血管 生长因子和激素 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GFI1B |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. Function: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. Subunit: Component of a RCOR-GFI-KDM1A-HDAC complex. Interacts directly with RCOR1, KDM1A and HDAC2 (By similarity). Forms a complex with GATA1. Interacts with histone methyltransferases EHMT2 and SUV39H1. Interacts with ARIH2 (via RING-type 2). Interacts with RUNX1T1. Subcellular Location: Nucleus. Tissue Specificity: Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA). Post-translational modifications: Methylation at Lys-8 in the SNAG domain seems required for the recruitment of the corepressor complex. Similarity: Contains 6 C2H2-type zinc fingers. Database links: Entrez Gene: 8328 Human Omim: 604383 Human SwissProt: Q5VTD9 Human Unigene: 553160 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
红系和巨核系谱系发育和分化所必需的原癌基因转录调节因子。转录抑制物或激活剂依赖于启动子和细胞类型的上下文；抑制SOCS1和SOCS3的启动子活性，从而可以调节细胞因子信号转导通路。与GATA1协同抑制靶基因转录，如凋亡调节因子BCL2L1；白血病细胞系GFI1b沉默显著增加细胞凋亡率。抑制IL6处理的骨髓单核细胞中MYC和MYB以及细胞周期蛋白依赖性激酶抑制剂CDKN1A/P21WAF1的下调。抑制GATA3在T细胞淋巴瘤中的表达，抑制GATA1介导的转录；当GATA1也介导红系GFI1b转录时，GATA1和GFI1b参与调控红细胞中GFI1b基因表达的反馈调控途径。通过蛋白质相互作用抑制GATA1介导的GFI1b启动子的刺激。绑定到伽玛卫星DNA和它自己的启动子，自动压制它自己的表达。通过将组蛋白甲基转移酶招募到靶基因启动子来改变组蛋白甲基化。在异染色质形成中起重要作用。