人剪切应力和机械传导定量PCR芯片试剂盒
产品名称: 人剪切应力和机械传导定量PCR芯片试剂盒
英文名称: GQH-SSM
产品编号: GK024
产品价格: 0
产品产地: 美国
品牌商标: sciencell
更新时间: 2024-11-14T09:23:14
使用范围: null
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Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21
种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。
确保了实验结果的重复性和连贯性。
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人剪切应力和机械传导定量PCR芯片试剂盒描述:
ScienCells GeneQuery™ Human Shear Stress and Mechanotransduction qPCR array kit (GQH-SSM) is designed to facilitate gene expression profiling of 88 key endothelial cell genes responsive to shear stress and mechanotransduction. Brief examples of how included genes may be grouped according to function are shown below:
- Signal transduction: CAV1, IP3R, MAPK1, PIAS1, SENP2
- ECM/cytoskeletal remodeling: COL4A2, DNM1, LAMA4, PMP22, VCAM1
- Immune response: IL13RA1, NFKB1, SELE, PTGS2, TLR2
- Transcription factors: GATA4, NCOR2, SP1, TRA2B, PPARG
- Ion transport: CCL14, NUP62, PIEZO1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Shear Stress and Mechanotransduction qPCR Array Plate Layout* (8 controls in Bold and Italic)
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
A2M |
CCL14 |
DUOX1 |
GATA3 |
KLF4 |
MFAP2 |
NUP62 |
PIK3CA |
PTK2 |
SLC9A3R2 |
TAGLN |
β-actin |
B |
ACTA2 |
CCL2 |
ECH1 |
GATA4 |
KRAS |
MT1A |
OLR1 |
PKD1 |
QDPR |
SOD1 |
TLR2 |
GAPDH |
C |
ADAM15 |
CCNC |
EDN3 |
GPC1 |
LAMA4 |
MYH10 |
P2RX1 |
PLAU |
RHOA |
SOD2 |
TOP1 |
LDHA |
D |
AKT1 |
CDK2 |
EFNA1 |
HMOX1 |
MAP1B |
NCOR2 |
P2RX4 |
PPARA |
SAE1 |
SP1 |
TRA2B |
NONO |
E |
BBC3 |
COL4A2 |
EGR1 |
IL13RA1 |
MAPK1 |
NDRG1 |
P2RX5 |
PPARG |
SELE |
SRC |
TYMS |
PPIH |
F |
BDKRB2 |
CYBB |
ELF2 |
ITPR3 |
MAPK7 |
NFKB1 |
PIAS1 |
PRKCE |
SENP1 |
SREBF1 |
UBA2 |
GDC |
G |
BIRC2 |
CYC1 |
FABP1 |
JAK1 |
MDM2 |
NOX1 |
PIAS3 |
PTGIS |
SENP5 |
SUMO2 |
UBE2I |
PPC |
H |
CAV1 |
DNM1 |
GADD45A |
JUN |
MEF2A |
NOX5 |
PIEZO1 |
PTGS2 |
SLC3A2 |
SUMO3 |
VCAM1 |
NTC |
* gene selection may be updated based on new research and development
Catalog No. | GK024 |
---|---|
Country of Manufacture | United States |
Product Code | GQH-SSM |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | GQH-SSM is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |