BstC8I内切酶-RNAi技术-试剂-生物在线

产品名称： BstC8I内切酶

英文名称： BstC8I

产品编号： HZ2207

产品价格： 0

产品产地：中国/上海

品牌商标：沪震生物

更新时间： 2023-08-17T10:24:20

使用范围： null

上海沪震实业有限公司

进入展台

BstC8I内切酶

中文名称：BstC8I内切酶
英文名称：BstC8I
产品规格：100U
储存条件：冷冻（-20℃）

使用说明

At 37℃ activity is 50%from maximum.

技术规格

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 55℃ in a total reaction volume of 50μl.
Recognition sites	GCN↑NGC
来源	Bacillus stearothermophilus C8
Assayed on	Lambda DNA
Optimal buffer	Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	55℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;10 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 55℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	Cac8I

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！