红细胞生成素受体(EPOR)重组蛋白
产品名称: 红细胞生成素受体(EPOR)重组蛋白
英文名称: Recombinant Erythropoietin Receptor (EPOR)
产品编号: YB027Ra011
产品价格: 0
产品产地: 中国/美国
品牌商标: 钰博生物/Ybscience
更新时间: 2023-08-17T10:29:50
使用范围: null
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红细胞生成素受体(EPOR)重组蛋白
YB027Ra011
Recombinant Erythropoietin Receptor (EPOR)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
红细胞生成素受体(EPOR)重组蛋白[ PROPERTIES ]
Residues: Arg276~Ala496 (Accession # P19235),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Cell membrane;
Single-pass type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
红细胞生成素受体(EPOR)重组蛋白sarcosyl and preservative.
Predicted isoelectric point: 5.0
Predicted Molecular Mass: 55.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
红细胞生成素受体(EPOR)重组蛋白GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- RALKQ KIWPGIPSPE
SE F E G L F T T H KG N F Q LW LY Q ND G C LWW SP C TP F TE DP PA S LE V LS E R CWG
TMQAVEPGTD DEGPLLEPVG SEHAQDTYLV LDKWLLPRNP PSEDLPGPGG SVDIVAMDEG
SEASSCSSAL ASKPSPEGAS AASFEYTILD PSSQLLRPWT LCPELPPTPP HLKYLYLVVS
DSGISTDYSS GDSQGAQGGL SDGPYSNPYE NSLIPA
[ REFERENCES ]
1. Winkelmann J.C., et al. (1990) Blood 76:24-30. 2. Jones S.S., et al. (1990) Blood 76:31-35. 3. Ehrenman K., St John T. (1991) Exp. Hematol. 19:973-977. 4. Kirito K., et al. (2002) Blood 99:102-110.