前胶原赖氨酸-1,2-酮戊二酸-5-双加氧酶1(PLOD1)重组蛋白

YB256Hu014
Recombinant Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 前胶原赖氨酸-1,2-酮戊二酸-5-双加氧酶1(PLOD1)重组蛋白[ PROPERTIES ]
Residues: Gln636~Pro727
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q02809
Host: E. coli
Subcellular Location: Rough endoplasmic
reticulum membrane; Peripheral membrane protein;
Lumenal side.
Purity: >95%
 前胶原赖氨酸-1,2-酮戊二酸-5-双加氧酶1(PLOD1)重组蛋白Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 9.1
Predicted Molecular Mass: 14.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
 前胶原赖氨酸-1,2-酮戊二酸-5-双加氧酶1(PLOD1)重组蛋白rotein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QFDLA FVVRYKPDEQ PSLMPHHDAS TFTINIALNR VGVDYEGGGC RFLRYNCSIR
APRKGWTLMH PGRLTHYHEG LPTTRGTRYI AVSFVDP