Biotinylated Anti-Human sCD40 Ligand-抗体-抗体-生物在线
上海研卉生物科技有限公司
Biotinylated Anti-Human sCD40 Ligand

Biotinylated Anti-Human sCD40 Ligand

商家询价

产品名称: Biotinylated Anti-Human sCD40 Ligand

英文名称: Biotinylated Anti-Human sCD40 Ligand

产品编号: 500-P142Bt

产品价格: null

产品产地: USA

品牌商标: PEPROTECH

更新时间: null

使用范围: ELISA/WB

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产品编号:
500-P142Bt
来源:
Polyclonal Rabbit
制备:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsCD40L. Anti-Human sCD40L specific antibody was purified by affinity chromatography and then biotinylated.
免疫原:
E.coli derived Recombinant Human sCD40 Ligand (PeproTech catalog #310-02)
应用:
ELISA
Direct
To detect hsCD40L by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsCD40L.
Sandwich
To detect hsCD40L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human sCD40L (500-P142) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsCD40L.
Western Blot
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.