pTrcHis2B载体基本信息
出品公司:别名:质粒类型:表达水平:启动子:克隆方法:载体大小:5' 测序引物及序列:3' 测序引物及序列:载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
Invitrogen
pTrcHis2B, pTrcHis2 B
大肠杆菌蛋白表达载体
高拷贝
Trc
多克隆位点，限制性内切酶
4404 bp
pTrcHis-F: gaggtatatattaatgtatcg
pTrcHis-R:GATTTAATCTGTATCAGG
C-His, C-Myc
Ampicillin
Hosts: E.coli NM522, E.coli INValphaF'. Related vectors: pSE420.
V365-20
瞬时表达 Transient
组成型 Constitutive
非病毒
pTrcHis2B载体质粒图谱和多克隆位点信息



pTrcHis2B载体简介
The pTrcHis2 plasmids are pBR322-derived expression vectors designed for efficient recombinant protein expression and purification in E. coli. High levels of expression are possible using the trc (trp-lac) promoter (Egon et al., 1983) and the rrnB anti-termination region (Li et al., 1984). The trc promoter contains the –35 region of the trp promoter together with the –10 region of the lac promoter (Brosius et al., 1985; Egon et al., 1983; Mulligan et al., 1985). To regulate expression, the gene encoding Lac repressor (lacIq) is provided in the pTrcHis2 vectors, allowing regulation of the trc promoter regardless of whether the host strain contains a gene encoding the Lac repressor.

Isopropyl-Beta-D-thiogalactopyranoside (IPTG) is used to induce expression of your gene. Translation is enhanced by the bacteriophage T7 gene 10 translation enhancer and a minicistron that provides highly efficient translational restart into the open reading frame of the multiple cloning site. DNA inserts are positioned downstream and in frame with the initiation ATG and a C-terminal fusion peptide. The C-terminal peptide encodes the myc epitope and six histidine residues that function as a metal binding site in the expressed protein.
pTrcHis2B载体序列
LOCUS       pTrcHis2\B              4404 bp    DNA     circular SYN  7-JUL-2013
DEFINITION  pTrcHis2 B.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.biofeng.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTNAME|pTrcHis2 B|
COMMENT     VNTOAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="pTrcHis2 A"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    235..257
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     misc_feature    264..333
                     /vntifkey="21"
                     /label=rrnB_ATS
     misc_feature    370..390
                     /vntifkey="21"
                     /label=pTrcHis_fwd_primer
     misc_feature    383..409
                     /vntifkey="21"
                     /label=minicis
     misc_feature    complement(587..604)
                     /vntifkey="21"
                     /label=pBAD_rev_primer
     misc_feature    complement(587..604)
                     /vntifkey="21"
                     /label=pTrcHis_rev_primer
     terminator      637..794
                     /vntifkey="43"
                     /label=rrnB_terminator
     terminator      760..803
                     /vntifkey="43"
                     /label=rrnB_T1_terminator
     terminator      935..962
                     /vntifkey="43"
                     /label=rrnB_T2_terminator
     promoter        1004..1032
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            1074..1934
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             1074..1934
                     /vntifkey="4"
                     /label=ORF\frame\2
     rep_origin      2089..2708
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    3105..3127
                     /vntifkey="21"
                     /label=pGEX_3_primer
     misc_feature    3274..4365
                     /vntifkey="21"
                     /label=lacI
     CDS             3406..4365
                     /vntifkey="4"
                     /label=ORF\frame\3
BASE COUNT     1091 a      1098 c      1160 g      1055 t
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
      301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
      361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaa