无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白

YB358Mu011
Wingless Wingless Wingless Wingless Type MMTV Integration Integration Integration Integration Site Family, Family, Family, Family, Member 10A (WNT10A) (WNT10A) (WNT10A) (WNT10A)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
6th Edition (Revised in March, 2013)
无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Ser108~Pro343 (Accession # Q9GZT5),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted, extracellular
space, extracellular matrix. Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.1
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 53.0kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
无翅型MMTV整合位点家族成员10A(WNT10A)重组蛋白MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ
GPLGSEF- SSL ETRNKIPYES PIFSRGFRES AFAYAIAAAG VVHAVSNACA LGKLKACGCD
AS R R G DEEA F RRK L H R L Q L D AL Q R G KG LS H GVPE HPA L P T ASP G L Q DSW E
WGGCSPDMGF GERFSKDF LD SREPHRDIHA RMRL HNNRVG RQAVMENMRR
KCKCHGTSGS CQLKTCWQVT PEFRTVGALL RSRFHRATLI RPHNRNGGQL EPGPAGAPSP
APGAPGPRRR ASPADLVYFE KSP
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. van den Boogaard M.J., et al. (2012) J. Med. Genet. 49:327-331.
2. Galbraith R.L., et al. (2011) Nutr Cancer 63:558-564.
3. Wedgeworth E.K., et al. (2011) Acta Derm. Venereol. 91:346-347.
4. Castori M., et al. (2011) Clin. Genet. 79:92-95.