HEPES
N-2-羟乙基哌嗪-N’-2-乙基磺酸  
产品编号 规    格 价    格
H8090-25    25g    70.00
H8090-100   100g   220.00
说明：生物缓冲试剂，可用于配制蛋白提取缓冲液、细胞培养
       用缓冲液等。有效缓冲范围为pH6.8-8.2。
别名：4-(2-羟乙基)-1-哌嗪乙磺酸 ； 4-羟乙基哌嗪乙磺酸;
        4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid ；
分子式：C8H18N2O4S    分子量：238.30
CAS＃：7365-45-9
外观：白色晶性粉末
特性：纯度：>99%          pKa(25℃)：7.5
           有效pH范围：6.8-8.2
           溶解性：溶于水。
储存条件：室温

 补充用途：对细胞无毒性作用。它是一种氢离子缓冲剂，能较长时间控制恒定的pH范围。使用终浓度为10-50mmol/L，一般培养液内含20mmol/LHEPES即可达到缓冲能力。细胞培养常用10mmol/L。

参考文献：  《Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell》 作者:Ao Jiao, Feng Li, Chengshuo Zhang, Wu Lv, Baomin Chen, and Jialin Zhang 期刊:International Journal of Endocrinology 影响因子:2.287 PMID:  《miR‐377‐5p inhibits lung cancer cell proliferation, invasion, and cell cycle progression by targeting AKT1 signaling》 作者:Han Wu, HaiYan Liu, WenJie Liu, YongLi Shi, Dawei Bao 期刊:Journal of Cellular Biochemistry 影响因子:3.448 PMID:30485528  《Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells》 作者:Ming Li, Jiali Gao, Yan Tang, Meishuo Liu, Sijin Wu, Kunli Qu, Xiangyu Long, Huajun Li, Min Li, Yinhui Liu, Jieli Yuan, Lei Mao, Yu Liu, Xiliang Zheng, Erkang Wang, Jin Wang and Yongliang Yang 期刊:Frontiers in Spotlight 影响因子:4.259 PMID:29375374  《SCFAs-induced GLP-1 Secretion Links the Regulation of Gut Microbiome on Hepatic Lipogenesis in Chickens》 作者:Jianmei ZHANG, Yin shuang Sun, Liqin Zhao, Tiantian Chen, Meina Fan, Hong Chao Jiao, Jinpeng Zhao, Xiaojuan Wang, Fu chang Li, Haifang Li, Hai Lin 期刊:bioRxiv 影响因子: PMID: "《A hepatic stem cell vaccine is superior to an embryonic stem cell vaccine in the prophylaxis and treatment of murine hepatocarcinoma》 作者:Qi Zheng,Yichao Zheng,Jing Chen,Jia You,Yueyong Zhu,Yurui Liu,JiaJi Jiang 期刊:Oncology  Reports 影响因子:2.976 PMID:28098898"  《Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability》 作者:Yunjia Song,Aobo Ma,Jia Ning,Xue Zhong,Qian Zhang,Xu Zhang,Guang Hong,Ying Li,and Changyi Li 期刊:International journal of nanomedicine 影响因子:4.37 PMID:30425487  《康复新液对人皮肤成纤维细胞增殖作用的影响》 作者:刘荣荣，李丽娟  ，李玥王楠张海珠 期刊:大理大学学报 影响因子:1.416 PMID:"  《Application of a Strontium-Loaded, Phase-Transited Lysozyme Coating to a Titanium Surface to Enhance Osteogenesis and Osteoimmunomodulation》 作者:Xin Lu, Wenxin Zhang,Zihao Liu,Shiqing Ma, Yingchun Sun,Xudong Wu, Xu Zhang and Ping Gao 期刊:Medical Sciene Monitor 影响因子:1.98 PMID:30973161