双解丝蛋白1(TWF1)重组蛋白

YB410Hu011
Twinfilin Twinfilin Twinfilin Twinfilin 1 (TWF1)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
1th Edition (Revised in February, 2012)
 双解丝蛋白1(TWF1)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Twinfilin 1
Gene Names: TWF1
Size: 100µg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: Actin-binding protein involved in motile and morphological processes. Inhibits
actin polymerization, likely by sequestering G-actin. By capping the barbed ends of
filaments, it also regulates motility. Seems to play an important role in clathrin-mediated
endocytosis and distribution of endocytic organelles
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Cytoplasm ›  双解丝蛋白1(TWF1)重组蛋白cytoskeleton

Tissue Specificity: Specificity: Specificity: Specificity: Expressed at high levels in the colon, testis, ovary, prostate and
lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1~Ser252 (Accession # Q12792), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 30.26 kDa as determined by SDS-PAGE reducing conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 30.26 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
 双解丝蛋白1(TWF1)重组蛋白[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGS-MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVIG SYSQPSDSWD KDYDSFVLPL
LEDKQPCYIL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE VFGTVKEDVS
LHGYKKYLLS QSSPAPLTAA EEELRQIKIN EVQTDVGVDT KHQTLQGVAF PISREAFQAL EKLNNRQLNY
VQLEIDIKNE IIILANTTNT ELKDLPKRIP KDSARYHFFL YKHSHEGDYL ES
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Beeler JF, et al.(1994) Mol Cell Biol 14 (2): 982-988.
2. Rush J., et al. (2005) Nat. Biotechnol. 23:94-101. 3. Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162. 4. Palmgren S, et al.(2002) J. Cell. Sci. 115 (Pt 5): 881-886.
5. Vartiainen MK, et al.(2003) J. Biol. Chem. 278 (36): 34347-34355.
6. Moseley,J.B. et al. (2006) J Cell Sci. 119 (Pt 8):1547-1557.
7. Hassel S, et al. (2004) Proteomics 4 (5): 1346-1358.
8. Beeler J.F., et al. (1994) Mol. Cell. Biol. 14:982-988.