载体基本信息
出品公司:载体名称:质粒类型:表达水平:启动子:克隆方法:载体大小:5 测序引物及序列:3 测序引物及序列:载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
Beckwith Lab
pBad43, pBad 43
大肠杆菌蛋白表达载体
高
araC
多克隆位点，限制性内切酶
6179 bp
pBAD-F: ATGCCATAGCATTTTTATCC
pBAD-R: gatttaatctgtatcagg
/
壮观霉素
Beckwith实验室构建，来源于pBad22。表达启动子是arabinose Bad启动子。
--
瞬时表达 Transient
诱导表达
非病毒
载体质粒图谱和多克隆位点信息

 
载体简介
 
载体序列
LCUS       pBAD43 6179 bp  DNA     circular SYN 
DEFINITION  pBAD43 
ACCESSION    
KEYWORDS     
SOURCE       
  ORGANISM  other sequences; artificial sequences; vectors. 
COMMENT     This file is created by Vector NTI 
            http://www.biofeng.com/ 
COMMENT     VNTAUTHORNAME|biofeng.com| 
FEATURES             Location/Qualifiers 
     source          1..6179 
                     /organism="pBAD43" 
                     /mol_type="other DNA" 
     misc_feature    complement(191..1069) 
                     /label="AraC" 
     CDS             complement(191..1120) 
                     /label="ORF frame 2" 
     misc_feature    1099..1114 
                     /label="araO2" 
     misc_feature    1256..1267 
                     /label="araO1" 
     misc_feature    1298..1311 
                     /label="CAP_BS" 
     misc_feature    1303..1322 
                     /label="pBAD_fwd_primer" 
     misc_feature    1308..1346 
                     /label="AraI1I2" 
     promoter        1343..1371 
                     /label="ARA_promoter" 
     misc_feature    complement(1494..1511) 
                     /label="pTrcHis_rev_primer" 
     misc_feature    complement(1494..1511) 
                     /label="pBAD_rev_primer" 
     terminator      1544..1701 
                     /label="rrnB_terminator" 
     terminator      1842..1869 
                     /label="rrnB_T2_terminator" 
     promoter        1910..1938 
                     /label="AmpR_promoter" 
     promoter        complement(1950..1966) 
                     /label="M13_forward20_primer" 
     misc_feature    complement(1959..1981) 
                     /label="M13_pUC_fwd_primer" 
     gene            complement(2489..3499) 
                     /label="spect" 
                     /gene="spect"                      
     CDS             complement(2489..3499) 
                     /label="ORF frame 2"                      
     CDS             3429..3887 
                     /label="ORF frame 3"                      
     CDS             complement(4314..5264) 
                     /label="ORF frame 1"                      
     promoter        6135..6164 
                     /label="lac_promoter" 
                      
ORIGIN 
    1 CCTGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC 
   61 GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATCATCGA TGCATAATGT GCCTGTCAAA 
  121 TGGACGAAGC AGGGATTCTG CAAACCCTAT GCTACTCCGT CAAGCCGTCA ATTGTCTGAT 
  181 TCGTTACCAA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC 
  241 GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT 
  301 CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA 
  361 GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT 
  421 GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA 
  481 TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT 
  541 TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT 
  601 CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA 
  661 TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG 
  721 TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT 
  781 AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC 
  841 CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA 
  901 CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT 
  961 CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG 
 1021 CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC 
 1081 TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG 
 1141 TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT 
 1201 AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA 
 1261 GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT 
 1321 CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT 
 1381 ACCCGTTTTT TTGGGCTAGC AGGAGGAATT CACCATGGTA CCCGGGGATC CTCTAGAGTC 
 1441 GACCTGCAGG CATGCAAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA 
 1501 CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC 
 1561 GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT 
 1621 AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC 
 1681 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG 
 1741 TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG 
 1801 GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA 
 1861 TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT CTAAATACAT TCAAATATGT 
 1921 ATCCGCTCAT GAGACAATAA CCCTAGCTTA CTGGCCGTCG TTTTACAACG TCGTGACTGG 
 1981 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG 
 2041 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 
 2101 GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA 
 2161 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG 
 2221 CCAACACCCG CTGACGAGCT TAGTAAAGCC CTCGCTAGAT TTTAATGCGG ATGTTGCGAT 
 2281 TACTTCGCCA ACTATTGCGA TAACAAGAAA AAGCCAGCCT TTCATGATAT ATCTCCCAAT 
 2341 TTGTGTAGGG CTTATTATGC ACGCTTAAAA ATAATAAAAG CAGACTTGAC CTGATAGTTT 
 2401 GGCTGTGAGC AATTATGTGC TTAGTGCATC TAACGCTTGA GTTAAGCCGC GCCGCGAAGC 
 2461 GGCGTCGGCT TGAACGAATT GTTAGACATT ATTTGCCGAC TACCTTGGTG ATCTCGCCTT 
 2521 TCACGTAGTG GACAAATTCT TCCAACTGAT CTGCGCGCGA GGCCAAGCGA TCTTCTTCTT 
 2581 GTCCAAGATA AGCCTGTCTA GCTTCACGTA TGACGGGCTG ATACTGGGCC GGCAGGCGCT 
 2641 CCATTGCCCA GTCGGCAGCG ACCTCCTTCG GCGCGATTTT GCCGGTTACT GCGCTGTACC 
 2701 AAATGCGGGA CAACGTAAGC ACTACATTTC GCTCATCGCC AGCCCAGTCG GGCGGCGAGT 
 2761 TCCATAGCGT TAAGGTTTCA TTTAGCGCCT CAAATAGATC CTGTTCAGGA ACCGGATCAA 
 2821 AGAGTTCCTC CGCCGCTGGA CCTACCAAGG CAACGCTATG TTCTCTTGCT TTTGTCAGCA 
 2881 AGATAGCCAG ATCAATGTCG ATCGTGGCTG GCTCGAAGAT ACCTGCAAGA ATGTCATTGC 
 2941 GCTGCCATTC TCCAAATTGC AGTTCGCGCT TAGCTGGATA ACGCCACGGA ATGATGTCGT 
 3001 CGTGCACAAC AATGGTGACT TCTACAGCGC GGAGAATCTC GCTCTCTCCA GGGGAAGCCG 
 3061 AAGTTTCCAA AAGGTCGTTG ATCAAAGCTC GCCGCGTTGT TTCATCAAGC CTTACGGTCA 
 3121 CCGTAACCAG CAAATCAATA TCACTGTGTG GCTTCAGGCC GCCATCCACT GCGGAGCCGT 
 3181 ACAAATGTAC GGCCAGCAAC GTCGGTTCGA GATGGCGCTC GATGACGCCA ACTACCTCTG 
 3241 ATAGTTGAGT CGATACTTCG GCGATCACCG CTTCCCTCAT GATGTTTAAC TTTGTTTTAG 
 3301 GGCGACTGCC CTGCTGCGTA ACATCGTTGC TGCTCCATAA CATCAAACAT CGACCCACGG 
 3361 CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG TACCCCAAAA AAACAGTCAT 
 3421 AACAAGCCAT GAAAACCGCC ACTGCGCCGT TACCACCGCT GCGTTCGGTC AAGGTTCTGG 
 3481 ACCAGTTGCG TGAGCGCATA CGCTACTTGC ATTACAGCTT ACGAACCGAA CAGGCTTATG 
 3541 TCCACTGGGT TCGTGCCTTC ATCCGTTTCC ACGGTGTGCG TCACCCGGCA ACCTTGGGCA 
 3601 GCAGCGAAGT CGAGGCATTT CTGTCCTGGC TGGCGAACGA GCGCAAGGTT TCGGTCTCCA 
 3661 CGCATCGTCA GGCATTGGCG GCCTTGCTGT TCTTCTACGG CAAGGTGCTG TGCACGGATC 
 3721 TGCCCTGGCT TCAGGAGATC GGAAGACCTC GGCCGTCGCG GCGCTTGCCG GTGGTGCTGA 
 3781 CCCCGGATGA AGTAATTCCC ACGGGTTTTG CTGCCCGCAA ACGGGCTGTT CTGGTGTTGC 
 3841 TAGTTTGTTA TCAGAATCGC AGATCCGGCT TCAGGTTTGC CGGCTGAAAG CGCTATTTCT 
 3901 TCCAGAATTG CCATGATTTT TTCCCCACGG GAGGCGTCAC TGGCTCCCGT GTTGTCGGCA 
 3961 GCTTTGATTC GATAAGCAGC ATCGCCTGTT TCAGGCTGTC TATGTGTGAC TGTTGAGCTG 
 4021 TAACAAGTTG TCTCAGGTGT TCAATTTCAT GTTCTAGTTG CTTTGTTTTA CTGGTTTCAC 
 4081 CTGTTCTATT AGGTGTTACA TGCTGTTCAT CTGTTACATT GTCGATCTGT TCATGGTGAA 
 4141 CAGCTTTGAA TGCACCAAAA ACTCGTAAAA GCTCTGATGT ATCTATCTTT TTTACACCGT 
 4201 TTTCATCTGT GCATATGGAC AGTTTTCCCT TTGATATGTA ACGGTGAACA GTTGTTCTAC 
 4261 TTTTGTTTGT TAGTCTTGAT GCTTCACTGA TAGATACAAG AGCCATAAGA ACCTCAGATC 
 4321 CTTCCGTATT TAGCCAGTAT GTTCTCTAGT GTGGTTCGTT GTTTTTGCGT GAGCCATGAG 
 4381 AACGAACCAT TGAGATCATA CTTACTTTGC ATGTCACTCA AAAATTTTGC CTCAAAACTG 
 4441 GTGAGCTGAA TTTTTGCAGT TAAAGCATCG TGTAGTGTTT TTCTTAGTCC GTTATGTAGG 
 4501 TAGGAATCTG ATGTAATGGT TGTTGGTATT TTGTCACCAT TCATTTTTAT CTGGTTGTTC 
 4561 TCAAGTTCGG TTACGAGATC CATTTGTCTA TCTAGTTCAA CTTGGAAAAT CAACGTATCA 
 4621 GTCGGGCGGC CTCGCTTATC AACCACCAAT TTCATATTGC TGTAAGTGTT TAAATCTTTA 
 4681 CTTATTGGTT TCAAAACCCA TTGGTTAAGC CTTTTAAACT CATGGTAGTT ATTTTCAAGC 
 4741 ATTAACATGA ACTTAAATTC ATCAAGGCTA ATCTCTATAT TTGCCTTGTG AGTTTTCTTT 
 4801 TGTGTTAGTT CTTTTAATAA CCACTCATAA ATCCTCATAG AGTATTTGTT TTCAAAAGAC 
 4861 TTAACATGTT CCAGATTATA TTTTATGAAT TTTTTTAACT GGAAAAGATA AGGCAATATC 
 4921 TCTTCACTAA AAACTAATTC TAATTTTTCG CTTGAGAACT TGGCATAGTT TGTCCACTGG 
 4981 AAAATCTCAA AGCCTTTAAC CAAAGGATTC CTGATTTCCA CAGTTCTCGT CATCAGCTCT 
 5041 CTGGTTGCTT TAGCTAATAC ACCATAAGCA TTTTCCCTAC TGATGTTCAT CATCTGAGCG 
 5101 TATTGGTTAT AAGTGAACGA TACCGTCCGT TCTTTCCTTG TAGGGTTTTC AATCGTGGGG 
 5161 TTGAGTAGTG CCACACAGCA TAAAATTAGC TTGGTTTCAT GCTCCGTTAA GTCATAGCGA 
 5221 CTAATCGCTA GTTCATTTGC TTTGAAAACA ACTAATTCAG ACATACATCT CAATTGGTCT 
 5281 AGGTGATTTT AATCACTATA CCAATTGAGA TGGGCTAGTC AATGATAATT ACTAGTCCTT 
 5341 TTCCTTTGAG TTGTGGGTAT CTGTAAATTC TGCTAGACCT TTGCTGGAAA ACTTGTAAAT 
 5401 TCTGCTAGAC CCTCTGTAAA TTCCGCTAGA CCTTTGTGTG TTTTTTTTGT TTATATTCAA 
 5461 GTGGTTATAA TTTATAGAAT AAAGAAAGAA TAAAAAAAGA TAAAAAGAAT AGATCCCAGC 
 5521 CCTGTGTATA ACTCACTACT TTAGTCAGTT CCGCAGTATT ACAAAAGGAT GTCGCAAACG 
 5581 CTGTTTGCTC CTCTACAAAA CAGACCTTAA AACCCTAAAG GCTTAAGTAG CACCCTCGCA 
 5641 AGCTCGGGCA AATCGCTGAA TATTCCTTTT GTCTCCGACC ATCAGGCACC TGAGTCGCTG 
 5701 TCTTTTTCGT GACATTCAGT TCGCTGCGCT CACGGCTCTG GCAGTGAATG GGGGTAAATG 
 5761 GCACTACAGG CGCCTTTTAT GGATTCATGC AAGGAAACTA CCCATAATAC AAGAAAAGCC 
 5821 CGTCACGGGC TTCTCAGGGC GTTTTATGGC GGGTCTGCTA TGTGGTGCTA TCTGACTTTT 
 5881 TGCTGTTCAG CAGTTCCTGC CCTCTGATTT TCCAGTCTGA CCACTTCGGA TTATCCCGTG 
 5941 ACAGGTCATT CAGACTGGCT AATGCACCCA GTAAGGCAGC GGTATCATCA ACAGGCTTAC 
 6001 CCGTCTTACT GTCGGGAATT AATTCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG 
 6061 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT 
 6121 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAG 
//
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载体应用举例