70kDa热休克蛋白14(HSPA14)重组蛋白

YB503Hu012
Heat Shock 70kDa Protein Protein Protein Protein 14 (HSPA14) (HSPA14) (HSPA14) (HSPA14)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
70kDa热休克蛋白14(HSPA14)重组蛋白[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Met1~Ser509 (Accession # Q0VDF9),
with N-terminal His-Tag.
Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm, cytosol.
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in 70kDa热休克蛋白14(HSPA14)重组蛋白PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.8
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 56.0kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG S -MAAIGVH LGC TSACVAVYKD GRAGVVANDA GDRVTPAVVA
YSENEEIVGL AAKQSRIRNI SNTVMKVKQI LGRSSSDPQA QKYIAESKCL VIEKNGKLRY
70kDa热休克蛋白14(HSPA14)重组蛋白EIDTGEETKF VNPEDVARLI FSKMKETAHS VLGSDANDVV ITVPFDFGEK QKNALGEAAR
AAGFNVLRLI HEPSAALLAY GIGQDSPTGK SNILVFKLGG TSLSLSVMEV NSGIYRVLST
NTDDNIGGAH FTETLAQYLA SEFQRSFKHD VRGNARAMMK LTNSAEVAKH SLSTLGSANC
FLDSLYEGQD FDCNVSRARF ELLCSPLFNK CIEAIRGLLD QNGFTADDIN KVVLCGGSSR
IPKLQQLIKD LFPAVELLNS IPPDEVIPIG AAIEAGILIG KENLLVEDSL MIECSARDIL
VKGVDESGAS RFTVLFPSGT PLPARRQHTL QAPGSISSVC LELYESDGKN SAKEETKFAQ
VVLQDLDKKE NGLRDILAVLTMKRDGSLHV TCTDQETGKC EAISIEIAS