人上皮细胞转化序列2(ECT2)多克隆抗体
Polyclonal Antibody to Epithelial Cell Transforming Sequence 2 (ECT2)
[PRODUCT INFORMATION]
Immunogen: ECT2, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant ECT2 (Leu166~Arg348) with N-terminal His-Tagexpressed in E.coli.
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against ECT2. It has beenselected for its ability to recognize ECT2 in immunohistochemical staining andwestern blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[QUALITY CONTROL ] 
Content:The quality control contains recombinant ECT2 (Leu166~Arg348)disposed in loading buffer
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[IMAGES ]


Used in Western Blot, Sample:
Lane1: Human lung Tissue
Lane2: Porcine Brain Tissue
Lane3: 293T Cells
Lane4: A549 Cells
Primary Ab: 2µg/mL Rabbit Anti-Human ECT2 Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。