小鼠含KH域RNA结合信号通路关联蛋白1(KHDRBS1)多克隆抗体
Polyclonal Antibody to KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
[ IMMUNOGEN ]
Immunogen:Recombinant KHDRBS1 (Leu156~Glu381) expressed in E.coli
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end use
[ FORMULATION ]
Form & Buffer:Supplied as solution form in 0.01M PBS, pH7.4, containing0.05% Proclin-300, 50% glycerol.
[QUALITY CONTROL ] 
Content:The quality control contains recombinant KHDRBS1 disposed in loadingbuffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, 0.02%NaN₃ 
[ STORAGE AND STABILITY ] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ IDENTIFICATION ]

Figure 1. Western Blot
A. Sample: Recombinant KHDRBS1, Mouse B. Lane1: Mouse Brain Tissue Lane2: Mouse Large Intestine Tissue Lane3: Human Hela Cells Primary Ab: 3µg/mL Rabbit Anti-Mouse KHDRBS1 Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig

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