人着丝粒蛋白I(CENPI)多克隆抗体
Polyclonal Antibody to Centromere Protein I (CENPI)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
[ IMMUNOGEN ]
Immunogen:Recombinant CENPI (Leu292~Gly528) expressed in E.coli
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer:Supplied as solution form in 0.01M PBS, pH7.4, containing0.05% Proclin-300, 50% glycerol.
[QUALITY CONTROL ] 
Content:The quality control contains recombinant CENPI disposed in loadingbuffer
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN₃ 
[ STORAGE AND STABILITY ] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ IDENTIFICATION ]

Figure 1. Western Blot
A. Sample: Recombinant CENPI, Human B. Human Raji Cells Primary Ab: 2µg/mL Rabbit Anti-Human CENPI Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig

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