棕榈酰蛋白硫酯酶1(PPT1)重组蛋白

YB621Mu012
Recombinant Palmitoyl Protein Thioesterase 1 (PPT1)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 棕榈酰蛋白硫酯酶1(PPT1)重组蛋白[ PROPERTIES ]
Residues: Asp28~Lys279
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O88531
Host: E. coli
Subcellular Location: Lysosome.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 8.1
Predicted Molecular Mass: 32.3kDa
 棕榈酰蛋白硫酯酶1(PPT1)重组蛋白Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 棕榈酰蛋白硫酯酶1(PPT1)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
DPP SPPPLVIWHG MGDSCCNPMS MGVIKKMVEK EIPGIYVLSL EIGKNMMEDV
ENSFFLNVNV QVNMVCQILE KDPKLQQGYN AIGFSQGGQF LRAVAQRCPT PPMMTLISVG
GQHQGVFGLP RCPGESSHIC DFIRKSLNAG AYSKLVQERL VQAQYWHDPI KESVYRNYSI
FLADINQERC VNESYKKNLM ALKKFVMVKF FNDSIVDPVD SEWFGFYRSG QAKETIPLQE
STLYTEDRLG LKKMDKAGK
[ REFERENCES ]
1. Salonen T., et al. (1998) Genome Res. 8:724-730. 2. Zhang Z., et al. (1999) Gene 231:203-211. 3. Ahtiainen L., et al. (2003) J. Comp. Neurol. 455:368-377. 4. Bible E., et al. (2004) Neurobiol. Dis. 16:346-359.