Frequently Asked Questions

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Analysis Note

≥98% SDS-PAGE

Biochem/physiol Actions

Qualified for use as a mitogen for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Application

Qualified for use as a mitogen for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.

General description

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids. Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion. EGF is a member of a growth factor family which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF. EGF is structurally homologous to human transforming growth factor-α (TGF-alpha), and both exert their actions through EGF receptors. EGF is homologous to a sequence contained in a 19 kD protein of vaccinia virus, which appears to utilize the EGF receptor to gain entry into cells.

EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.

法律信息

LONG 注册商标 Novozymes Gropep Limited

Other Notes

The biological activity of recombinant, human EGF is measured by its ability to stimulate the mouse fibroblast cell line, BALB/3T3. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.