免疫球蛋白G4(IgG4)重组蛋白

YB234Hu014
Recombinant Immunoglobulin G4 (IgG4)Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
免疫球蛋白G4(IgG4)重组蛋白Residues: Gln222~Lys327
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P01861
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 100mM
NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA,
免疫球蛋白G4(IgG4)重组蛋白1mM DTT, 0.01% sarcosyl, 5% trehalose, and
preservative.
Predicted isoelectric point: 6.5
Predicted Molecular Mass: 15.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
免疫球蛋白G4(IgG4)重组蛋白than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QPREPQVYT LPPSQEEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS
DGSFFLYSRL TVDKSRWQEG NVFSCSVMHE ALHNHYTQKS LSLSLGK
[ REFERENCES ]
1. Ellison J.W., et al. (1981) DNA 1:11-18. 2. Pink J.R.L., et al. (1970) Biochem. J. 117:33-47. 3. Chen R., et al. (2009) J. Proteome Res. 8:651-661. 4. Davies A.M., et al. (2013) Mol. Immunol. 54:1-7.