phospho-NEK9(Thr210),磷酸化丝氨酸/苏氨酸蛋白激酶NEK9抗体

产品编号HZ-9318R
英文名称phospho-NEK9(Thr210)
中文名称磷酸化丝氨酸/苏氨酸蛋白激酶NEK9抗体
别 名NEK9 (phospho Thr210); p-NEK9 (Thr210); NEK9 (phospho T210); p-NEK9 (T210); Nek 8; NEK 9; Nek8; nek9; Nek-8; nek-9; NEK9_HUMAN; NERCC 1; Nercc 1 kinase; NERCC; NERCC1; Nercc1 kinase; Never in mitosis A related kinase 9; Never in mitosis A-related kinase 9; NIMA (never in mitosis gene a) related kinase 9; NIMA related kinase 8; NIMA related kinase 9; NIMA related kinase Nek 8; NIMA related kinase Nek 9; NIMA related kinase Nek8; NIMA related kinase Nek9; NimA related protein kinase 9; NIMA-related kinase 8; NimA-related protein kinase 9; Serine/threonine protein kinase Nek 9; Serine/threonine protein kinase Nek9; Serine/threonine-protein kinase Nek9.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 信号转导 细胞周期蛋白 激酶和磷酸酶 细胞分化
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
phospho-NEK9(Thr210),磷酸化丝氨酸/苏氨酸蛋白激酶NEK9抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量107kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human NEK9 around the phosphorylation site of Thr210
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-NEK9(Thr210),磷酸化丝氨酸/苏氨酸蛋白激酶NEK9抗体PubMedPubMed
产品介绍background:
Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.

Function:
Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.

Subunit:
Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

Post-translational modifications:
Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. [SIMILARITY] Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. [SIMILARITY] Contains 1 protein kinase domain.

Similarity:
Contains 6 RCC1 repeats.

Gene ID:
91754

phospho-NEK9(Thr210),磷酸化丝氨酸/苏氨酸蛋白激酶NEK9抗体Database links:
Entrez Gene: 91754 Human
Entrez Gene: 217718 Mouse
Entrez Gene: 299204 Rat
Omim: 609798 Human
SwissProt: Q8TD19 Human
SwissProt: Q8K1R7 Mouse
Unigene: 7200 Human
Unigene: 29071 Mouse
Unigene: 102369 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.