小鼠多萜醇二磷酸寡糖蛋白环糊精糖基转移酶(DDOST)多克隆抗体

Polyclonal Antibody to Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST)

[ PRODUCT INFORMATION ]

Immunogen: DDOST, Mouse

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200µg/mL

UOM: 100µg

[ IMMUNOGEN INFORMATION ]

Recombinant DDOST (Gly29~Pro412) expressed in E.coli.

Sequence: The target protein is fused with N-terminal His-Tag and its sequenceis listed below.

MGHHHHHHSG SEF-GP RTLVLLDNLN VRDTHSLFFR SLKDRGFELT FKTADDPSLSLIKYGEFLYD NLIIFSPSVE DFGGNINVET ISAFIDGGGS VLVAASSDIG DPLRELGSECGIEFDEEKTA VIDHHNYDVS DLGQHTLIVA DTENLLKAPT IVGKSSLNPI LFRGVGMVADPDNPLVLDIL TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV IFSGSLDFFSDA F F NS AV QK AT PGA Q RY SQ TG NYE LAVA L SRW VF KE EGV LRV GPV S H H RVGEMAPPNAY TVTDLVEYSI IIEQLSNGKW VPFDGDDIQL EFVRIDPFVR TFLKRKGGKYSVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH TQYERFIPSA YP

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against DDOST. It has beenselected for its ability to recognize DDOST in immunohistochemical staining andwestern blotting

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ] 

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.

[ QUALITY CONTROL ] 

Content:The quality control contains recombinant DDOST (Gly29~Pro412)disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%

[ STORAGE ] 

Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。