pET49b载体基本信息
出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列:  3' 测序引物:3' 测序引物序列:  载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
EMD Biosciences (Novagen)
pET49b, pET 49b
大肠杆菌蛋白表达
高
多克隆位点，限制性内切酶
5926 bp
T7或者pGEX 5'
T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX 5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
T7t
T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
N-GST, N-His, N-HRV 3C, C-S, C-Thrombin
Kanamycin
Same as pET47 but also has Nterm GST Tag; contains HRV 3C Protease cleavage site for fusion tag removal at low temperatures; Cterm thrombin cleavage site.
71463-3
瞬时表达 Transient
组成型 Constitutive
非病毒
pET49b载体质粒图谱和多克隆位点信息


pET49b载体序列
LOCUS       pET-49b(+)              5926 bp    DNA     circular     11-SEP-2008
DEFINITION  pET-49b(+).
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     rep_origin      complement(5467..5914)
                     /vntifkey="33"
                     /label=f1\ori
     misc_RNA        complement(3843..4398)
                     /vntifkey="53"
                     /label=RNA\II
     rep_origin      complement(3843..3843)
                     /vntifkey="33"
                     /label=ColE1,\pBR\ori
     misc_RNA        4283..4390
                     /vntifkey="53"
                     /label=RNA\I
     CDS             3221..3412
                     /vntifkey="4"
                     /label=ROP\gene
     CDS             1567..2649
                     /vntifkey="4"
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1160..1176)
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    complement(1135..1155)
                     /vntifkey="31"
                     /label=lacO
     CDS             complement(429..1088)
                     /vntifkey="4"
                     /label=GST
     protein_bind    complement(342..359)
                     /vntifkey="31"
                     /label=His-Tag
     terminator      complement(26..73)
                     /vntifkey="43"
                     /label=T7\terminator
     CDS             4552..5367
                     /vntifkey="4"
                     /label=Kan
     misc_feature    complement(213..230)
                     /vntifkey="21"
                     /label=Thrombin\site
     protein_bind    complement(168..212)
                     /vntifkey="31"
                     /label=S-Tag
     protein_bind    complement(310..332)
                     /vntifkey="31"
                     /label=3c
     primer          complement(300..336)
                     /vntifkey="27"
                     /label=S-SacII/Bam\+SanDI\-dcm
     primer          complement(374..421)
                     /vntifkey="27"
                     /label=S-Tag\Spacer-Spe\I
BASE COUNT     1485 a      1486 c      1520 g      1435 t
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgtttagcag cctaggttaa ttaagcctcg agagcagcag aagtagagct gtccatgtgc
      181 tggcgttcga atttagcagc agcggtttct ttactaccgc gtggcaccag agcgagctct
      241 gcggccgcaa gcttgtcgac ggacgtcggg cgcgccaagg cctgtacaga attcggatcc
      301 tggtacccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg atggtgatga
      361 ccagaaccac tagatggagt aggagtagga ggattgttat tagaaccgcc accaccacta
      421 gttgaaccat ccgattttgg aggatggtcg ccaccaccaa acgtggcttg ccagccctgc
      481 aaaggccatg ctatatactt gctggatttc aagtacttat caatttgtgg gatagcttca
      541 atacgttttt taaaacaaac taattttggg aacgcatcca ggcacattgg gtccatgtat
      601 aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg atcaccattt
      661 aaatatgttt tatgacataa acgatcttcg aacattttca gcatttcagg tagcttgcta
      721 agaaaatcaa ctttgagagt ttcaaagtct ttactatatg caattctcga aacaccgtat
      781 ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg cacgctcttt tggacaacca
      841 cccaacatgt tgtgcttgtc agctatataa cgtatgatgg ccatagactg tgttaattta
      901 acatcaccat caatataata aggaagattg ggaaactcca aacccaattc aaactttttg
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