14-3-3 Family Antibody Sampler Ki该试剂盒包含充足的一抗和二抗，且每种抗体能够进行四种免疫印迹（Western blot）反应。

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody.

Western Blotting

Western blot analysis of extracts from various cell types using 14-3-3 ε Antibody.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody #9638.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody #9636.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, PC12, and COS cells using 14-3-3 ε Antibody #9635.

Western Blotting

Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb.

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb.

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb.

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb #5522.

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb #7413.

Western Blotting

Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb #5521.

Background

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes which modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).

14-3-3蛋白家族在信号转导、关卡调控 （ checkpoint control）、凋亡和养分敏感通路中起着关键性调节作用(1,2)。14-3-3蛋白是高度保守且广泛表达的蛋白。至少七种亚型包括β、γ、ε、σ、ζ、τ和η ，它们都在哺乳动物体内被鉴定。最初描述的α和δ 亚型是分别被确认为 β和ζ亚型的磷酸化形式(3)。通过它们的氨基末端α 螺旋区域，14-3-3蛋白形成同源或异源二聚体，这些二聚体与很多的蛋白质包括转录因子、新陈代谢酶、细胞支架 蛋白、激酶、磷酸化酶和其它信号分子相互作用(3,4)。主要通过phospho-Ser/Thr motif促使14-3-3蛋白与它们的靶蛋白相互作用。然而，结合不同的phospho-Ser/Thr motifs和非磷酸化依赖的相互作用一样都已经被观察(4)。14-3-3蛋白的结合掩饰了其靶蛋白特异性序列，因此，该蛋白调节靶蛋白的定位、磷酸化水平、稳定性和分子相互作用(1-4)。14-3-3蛋白也可能诱导靶蛋白构象的改变，从而可调节靶蛋白功能(4,5)。明显的14-3-3蛋白亚型的时空表达模式在对细胞外信号和药物的发展和急性期已经被观察，这就认为尽管14-3-3蛋白亚型的序列相似，但是可能具有不同的功能(4)。数个研究认为14-3-3 蛋白亚型在癌症和神经综合征中不同的被调节(2,3)。

12630 SignalFire™ Plus ECL Reagent

12757 SignalFire™ Elite ECL Reagent

6883 SignalFire™ ECL Reagent

7074 Anti-rabbit IgG, HRP-linked Antibody

7720 Prestained Protein Marker, Broad Range (Premixed Format)

7727 Biotinylated Protein Ladder Detection Pack