pACYCDuet™-1 is designed for the coexpression of two target genes. The vector
contains two multiple cloning sites (MCS), each of which is preceded by a T7
promoter/lac operator and ribosome binding site (rbs). The vector also carries the P15A
replicon, lacI gene and chloramphenicol resistance gene. This vector can be used in
combination with pETDuet™-1 in an appropriate host strain for the
coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using
the ACYCDuetUP1 Primer  and DuetDOWN1 Primer . Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer  and T7 Terminator Primer .