磷酸化丝裂原活化蛋白激酶激酶8抗体
产品名称： 磷酸化丝裂原活化蛋白激酶激酶8抗体
英文名称： Phospho-MAP3K8 (Thr290)
产品编号： hz-3455R
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
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Rabbit Anti-Phospho-MAP3K8 (Thr290) antibody
| 产品编号 | hz-3455R |
| 英文名称 | Phospho-MAP3K8 (Thr290) |
| 中文名称 | 磷酸化丝裂原活化蛋白激酶激酶8抗体 |
| 别 名 | MAP3K8 (Phospho T290); MAP3K8 (Phospho Thr290); P-MAP3K8/Tpl2 (Thr290); Phospho-Tpl2 (Thr290); P-Tpl2 (Thr290);c COT; Cancer Osaka thyroid oncogene; Cancer Osaka thyroid oncogene; CCOT; COT; COT proto oncogene serine/threonine protein kinase; EST; ESTF; Ewing sarcoma transformant; FLJ10486; M3K8_HUMAN; MAP3K 8; MAP3K8; Mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Proto oncogene cCot; Proto-oncogene c-Cot; Serine/threonine protein kinase cot; Serine/threonine-protein kinase cot; TPL 2; TPL-2; TPL2; Tumor progression locus 2. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 53kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human MAP3K8/Tpl2 around the phosphorylation site of Thr290:RG(p-T)EI |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]. Function: Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. Subunit: Forms a ternary complex with NFKB1 and TNIP2. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in several normal tissues and human tumor-derived cell lines. Post-translational modifications: Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. SWISS: P41279 Gene ID: 1326 Database links: Entrez Gene: 1326 Human Entrez Gene: 26410 Mouse Entrez Gene: 116596 Rat Omim: 191195 Human SwissProt: P41279 Human SwissProt: Q07174 Mouse SwissProt: Q63562 Rat Unigene: 432453 Human Unigene: 3275 Mouse Unigene: 9939 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
 Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-MAP3K8/Tpl2(Thr290) Polyclonal Antibody, Unconjugated(bs-3455R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |