上海中乔新舟生物科技有限公司是美国Sciencell公司在中国一级代理：

         美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。

请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品。

大鼠胚胎成纤维细胞
 

Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells [1]. REF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. REF are isolated from rat embryos and used at early passages [2]. To serve as feeder cells, REF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.

REF from ScienCell Research Laboratories are isolated from embryonic day 14 rat embryos. These cells are cryopreserved at P0 and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. REF are characterized by immunofluorescence with antibody specific to fibronectin. REF are negative for mycoplasma, bacteria, yeast, and fungi. REF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing REF in vitro.