phospho-IRE1a (Ser 726),磷酸化内质网核信号转导蛋白a1抗体

产品编号HZ-4308R
英文名称phospho-IRE1a (Ser 726)
中文名称磷酸化内质网核信号转导蛋白a1抗体
别 名p-IRE1a (phospho-Ser 726); IRE1 (phospho S726) ; IRE1 alpha (p-Ser726); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring protein 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; Protein kinase/endoribonuclease; Serine/threonine protein kinase/endoribonuclease IRE1.ERN1_HUMAN
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
phospho-IRE1a (Ser 726),磷酸化内质网核信号转导蛋白a1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量107kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human IRE1a around the phosphorylation site of Ser 726
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-IRE1a (Ser 726),磷酸化内质网核信号转导蛋白a1抗体PubMedPubMed
产品介绍background:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Function:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Subunit:
Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. Interacts with TAOK3 and TRAF2.

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Tissue Specificity:
Ubiquitously expressed. High levels observed in pancreatic tissue.

Post-translational modifications:
Autophosphorylated.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.

phospho-IRE1a (Ser 726),磷酸化内质网核信号转导蛋白a1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.