GLRB,甘氨酸受体β/GlyR β抗体

产品编号HZ-11559R
英文名称GLRB
中文名称甘氨酸受体β/GlyR β抗体
别 名Glycine receptor 58 kDa subunit; Glycine receptor beta; Glycine receptor subunit beta; Glycine receptor, beta subunit; GLRB_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 通道蛋白 细胞膜受体
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
GLRB,甘氨酸受体β/GlyR β抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量54kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human GLRB (23-110aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
GLRB,甘氨酸受体β/GlyR β抗体PubMedPubMed
产品介绍background:
GLRB (Glycine receptor beta) is a neurotransmitter-gated ion channel concentrated within the spinal cord and brainstem. Expression is also observed in several upper brain regions including the cortex, cerebellum, hippocampus and amygdala. Binding of glycine to GLRB increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing), controlling spinal reflexes and locomotor behavior.

Function:
The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).

Subunit:
Pentamer composed of alpha and beta subunits. Interacts with GPHN

Subcellular Location:
Plasma membrane; multi-pass membrane protein.

DISEASE:
Defects in GLRB are the cause of hyperekplexia type 2 (HKPX2) [MIM:614619]. HKPX2 is a neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile2 stimuli.

Similarity:
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRB sub-subfamily.

Gene ID:
2743

GLRB,甘氨酸受体β/GlyR β抗体Database links:
Entrez Gene: 2743 Human
Entrez Gene: 14658 Mouse
Entrez Gene: 25456 Rat
Omim: 138492 Human
SwissProt: P48167 Human
SwissProt: P48168 Mouse
SwissProt: P20781 Rat
Unigene: 32973 Human
Unigene: 275639 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-GLRB Polyclonal Antibody, Unconjugated(HZ-11559R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(HZ-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-GLRB Polyclonal Antibody, Unconjugated(HZ-11559R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining