乳腺癌易感蛋白2(BRCA2)重组蛋白

YB364Ra012
Recombinant Breast Cancer Susceptibility Protein 2 (BRCA2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Lys2308~His2537
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P51587
Host: E. coli
Purity: >95%
 乳腺癌易感蛋白2(BRCA2)重组蛋白Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.7
Predicted Molecular Mass: 30.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
 乳腺癌易感蛋白2(BRCA2)重组蛋白Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
 乳腺癌易感蛋白2(BRCA2)重组蛋白The sequence of the target protein is listed below.
KST PDGTIK DRR L FMHHVS LEPI TCVPFRT TKE RQEIQNPNFT APGQEFLSKS
HLYEHLTLEK SSSNLAVSGH PFYQVSATRN EKMRHLITTG RPTKVFVPPF KTKSHFHRVE
QCVRNINLEE NRQKQNIDGH GSDDSKNKIN DNEIHQFNKN NSNQAAAVTF TKCEEEPLDL
ITSLQNARDI QDMRIKKKQR QRVFPQPGSL YLAKTSTLPR ISLKAAVGGQ VPSACSH
[ REFERENCES ]
1. Schoenfeld A.R., et al. (2004) Mol. Cell. Biol. 24:7444-7455. 2. Wang X.Z., et al. (2004) Mol. Cell. Biol. 24:5850-5862. 3. Hussain S., et al. (2004) Hum. Mol. Genet. 13:1241-1248. 4. Ozcelik H., et al. (1997) Nat. Genet. 16:17-18.