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上海信裕生物科技有限公司
DIO1抗体

DIO1抗体

商家询价

产品名称： DIO1抗体

英文名称： DIO1Antibody

产品编号： XY 11790-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 DIO1抗体

Applications
In-house applications ELISA, WB, IHC, IP, IF     Application not tested? Please see our Guarantee.
Published applications IHC, WB Publications 2 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human, mouse, RAT
Images
Prev12NextView more validation data
  • 11790-1-AP;A549 cellA549 cells were subjected to SDS PAGE followed by western blot with 11790-1-AP(DIO1 antibody) at dilution of 1:1000
  • 11790-1-AP;human ovary tumor tissueImmunohistochemical of paraffin-embedded human ovary tumor using 11790-1-AP(DIO1 antibody) at dilution of 1:50 (under 10x lens)
  • 11790-1-AP;human ovary tumor tissueImmunohistochemical of paraffin-embedded human ovary tumor using 11790-1-AP(DIO1 antibody) at dilution of 1:50 (under 40x lens)
  • 11790-1-AP;A549 cellsIP Result of anti-DIO1 (IP:11790-1-AP, 4ug; Detection:11790-1-AP 1:1000) with A549 cells lysate 1200ug.
  • 11790-1-AP;A549 cellsImmunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 11790-1-AP( DIO1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in A549 cells, HeLa cells, U-937 cells
Positive IP detected in A549 cells
Positive IHC detected in Human ovary tumor tissue, human kidney tissue, human liver tissue
Positive IF detected in A549 cells
Recommended dilution WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:50-1:500
 DIO1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information DIO1抗体
Immunogen DIO1 fusion protein ag2410 Full name Deiodinase, iodothyronine, type I
Calculated molecular
weight		 249aa,29 kDa Observed molecular 
weight		 13 kDa
GenBank accession 
number		 BC017955 Gene ID (NCBI) 1733
Gene symbol DIO1 Synonyms 5DI, DIO1, DIOI, ITDI1, TXDI1, Type 1 DI, Type I 5 deiodinase
Background

DIO1(Type I iodothyronine deiodinase) is also named as ITDI1, TXDI1,5DI,DIOI and belongs to the iodothyronine deiodinase family.It is a membrane selenoprotein that catalyzes the deiodination of L-thyroxine to the biologically active thyroid hormone 3,3′,5-triiodothyronine.DIO1 is located in liver, kidney, and thyroid,which has both ORD and IRD activities(PMID: 9389495). It has 9 isoforms produced by alternative splicing with the MW of 29 kDa, 21 kDa, 13 kDa, 23 kDa, 19 kDa, 4 kDa, 13 kDa, 9 kDa and 10 kDa. DIO1抗体

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