生精蛋白Ⅰ(SEMG1)重组蛋白

YB185Hu014
Recombinant Semenogelin I (SEMG1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
生精蛋白Ⅰ(SEMG1)重组蛋白[ PROPERTIES ]
Residues: Gln24~Thr462
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P04279
Host: E. coli
Subcellular Location: Secreted.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
生精蛋白Ⅰ(SEMG1)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 9.3
Predicted Molecular Mass: 53.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
生精蛋白Ⅰ(SEMG1)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK GSFSIQYTYH VDANDHDQSR
KS Q Q Y D L NA L HK T TK S Q R H L GGS Q Q L L H NK QE G R D H DK S K GH F H RV VI H H
KGGK A HRG TQ NPSQD QG NSP SGKGISSQYS NTEE R LWV H G LSKEQ TSVSG
AQKGRKQ GGS QSSYV LQTEE LVA NKQQ RET KNS HQ NKGHY QNVVEV REE H
SSKVQTSLCP AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS
SSTEERRLHY GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS
SS T E E R R L H Y GE N G V Q K DV S QRSI Y S Q T E K LVA G K S Q I Q A P NP K QE PW H G
ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL
FT