大鼠表面活性物质关联蛋白A2(SPA2)多克隆抗体

Polyclonal Antibody to Surfactant Associated Protein A2 (SPA2)

[ PROPERTIES ]

Source: Polyclonal antibody preparation

Host: Rabbit

Purification: Antigen-specific Affinity Chromatography. Traits: Liquid

Concentration: 200µg/mL

UOM: 100µg

Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.

[ IMMUNOGEN ]

Immunogen: Recombinant SPA2 (Asn21~Phe248) expressed in E.coli.

[ APPLICATIONS ]

Western blotting: 0.5-2ug/ml

Immunocytochemistry in formalin fixed cells: 5-20ug/ml

Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml

Immunohistochemistry in paraffin section: 5-20ug/ml

Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml

Optimal working dilutions must be determined by end user.

[ FORMULATION ]

Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing0.05% Proclin-300, 50% glycerol.

[ CONTENTS ] 

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.

[ QUALITY CONTROL ] 

Content:The quality control contains recombinant SPA2 disposed in loadingbuffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%

[ STORAGE AND STABILITY ] 

Storage: Avoid repeated freeze/thaw cycles.

Store at 4ºC for frequent use.

Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ IDENTIFICATION ]

本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。